il 1β polyclonal antibody  (Proteintech)

Journal: Journal of Traditional and Complementary Medicine

Article Title: Chemical characterization of Jing Guan Fang and its application in alleviating coronavirus envelope protein-induced proinflammatory responses in vitro and in vivo

doi: 10.1016/j.jtcme.2025.12.003

Figure Lengend Snippet: JGF inhibits NO, IL-6, and TNF-α production in RAW264.7 and MH-S cells. The cells were treated with JGF (50, 100, 150, 300, 600 μg/mL), 2-E (0.1 μM), DXT (10 μM), or LPS (0.1 μg/mL) for 24 h. ( A ) Cell viability was evaluated using crystal violet. ( B ) NO production was measured using the Griess assay. ( C-D ) IL-6 ( C ) and TNF-α ( D ) levels were determined by ELISA. EC 50 was calculated by CompuSyn software. Data was presented as mean ± standard deviation (SD) for groups (n = 3). Significant differences are denoted as ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Article Snippet: Primary antibodies against IL-6 (Bioss, BS0379R, 1:500), TNF-α (Bioworld, BS1857, 1:300), and IL-1β (Bioss, BS6319R, 1:500) were applied overnight at room temperature.

Techniques: Griess Assay, Enzyme-linked Immunosorbent Assay, Software, Standard Deviation

Journal: Journal of Traditional and Complementary Medicine

Article Title: Chemical characterization of Jing Guan Fang and its application in alleviating coronavirus envelope protein-induced proinflammatory responses in vitro and in vivo

doi: 10.1016/j.jtcme.2025.12.003

Figure Lengend Snippet: JGF downregulates 2-E-induced iNOS and COX-2 in RAW264.7 and MH-S cells. Cells were treated with JGF (0, 50, 200 μg/mL) or 2-E (0.1 μM) for 24 h. ( A ) Protein levels of iNOS and COX-2 in macrophages were measured by Western blot. ( B-C ) Quantification of iNOS and COX-2 in cells without ( B ) and with ( C ) 2-E stimulation, calculated using ImageJ. Actin was used as the internal control. The non-detected data showed as – or ND. Data are presented as mean ± SD (n = 3). Significant differences are denoted as ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

Article Snippet: Primary antibodies against IL-6 (Bioss, BS0379R, 1:500), TNF-α (Bioworld, BS1857, 1:300), and IL-1β (Bioss, BS6319R, 1:500) were applied overnight at room temperature.

Techniques: Western Blot, Control

Journal: Journal of Traditional and Complementary Medicine

Article Title: Chemical characterization of Jing Guan Fang and its application in alleviating coronavirus envelope protein-induced proinflammatory responses in vitro and in vivo

doi: 10.1016/j.jtcme.2025.12.003

Figure Lengend Snippet: JGF inhibits 2-E-induced phosphorylation of STAT3 in RAW264.7 and MH-S cells. Cells were treated with JGF (0, 50, 200 μg/mL) or 2-E (0.1 μM) for 3 h. ( A ) Protein levels of phosphorylated JAK2 and STAT3 were measured by Western blot. ( B-C ) Quantification of phosphorylated JAK2 and STAT3 in cells without ( B ) and with ( C ) 2-E stimulation, calculated using ImageJ. Actin was used as the internal control. Data are presented as mean ± SD (n = 3). Significant differences are denoted as ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

Article Snippet: Primary antibodies against IL-6 (Bioss, BS0379R, 1:500), TNF-α (Bioworld, BS1857, 1:300), and IL-1β (Bioss, BS6319R, 1:500) were applied overnight at room temperature.

Techniques: Phospho-proteomics, Western Blot, Control

Journal: Journal of Traditional and Complementary Medicine

Article Title: Chemical characterization of Jing Guan Fang and its application in alleviating coronavirus envelope protein-induced proinflammatory responses in vitro and in vivo

doi: 10.1016/j.jtcme.2025.12.003

Figure Lengend Snippet: JGF inhibits 2-E-induced phosphorylation of ERK1/2 in RAW264.7 and MH-S cells. Cells were treated with JGF (0, 50, 200 μg/mL) or 2-E (0.1 μM) for 3 h. ( A ) Protein levels of phosphorylated JNK1/2, ERK1/2, p38, and p65 were measured by Western blot. ( B-C ) Quantification of phosphorylated JNK1/2, ERK1/2, p38, and p65 in cells without ( B ) and with ( C ) 2-E stimulation, calculated using ImageJ. Actin was used as the internal control. Data are presented as mean ± SD (n = 3). Significant differences are denoted as ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

Article Snippet: Primary antibodies against IL-6 (Bioss, BS0379R, 1:500), TNF-α (Bioworld, BS1857, 1:300), and IL-1β (Bioss, BS6319R, 1:500) were applied overnight at room temperature.

Techniques: Phospho-proteomics, Western Blot, Control

Journal: Journal of Traditional and Complementary Medicine

Article Title: Chemical characterization of Jing Guan Fang and its application in alleviating coronavirus envelope protein-induced proinflammatory responses in vitro and in vivo

doi: 10.1016/j.jtcme.2025.12.003

Figure Lengend Snippet: JGF reduces the 2-E-induced proinflammatory cytokines in vivo . ( A ) The experimental scheme for mouse exposure. ( B-F ) Levels of IL-6 ( B ), TNF-α ( C ), IFN-γ ( D ), IL-1β ( E ), and IL-12 ( F ) in lung tissue and serum were measured by ELISA. Data are presented as mean ± SD (n = 9 for serum, except DXT group n = 6; n = 6 for lung tissue, except DXT group n = 3) ( G ) Representative histological images of lung tissue stained with H&E and IHC images for IL-6, TNF-α, and IL-1β expression. ( H-J ) Quantification of IL-6 ( H ), TNF-α ( I ), and IL-1β ( J ) positive areas using ImageJ (n = 3). Significant differences between the control (CTL) group and other groups are denoted by ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Significant differences between the 2-E group and 2-E + JGF group are indicated by #p < 0.05, ##p < 0.01, ###p < 0.001.

Article Snippet: Primary antibodies against IL-6 (Bioss, BS0379R, 1:500), TNF-α (Bioworld, BS1857, 1:300), and IL-1β (Bioss, BS6319R, 1:500) were applied overnight at room temperature.

Techniques: In Vivo, Enzyme-linked Immunosorbent Assay, Staining, Expressing, Control

Journal: Experimental Neurobiology

Article Title: Electroacupuncture Attenuates Neuroinflammation and Postoperative Cognitive Dysfunction in Aged Rats by Suppressing the cGAS–STING Pathway

doi: 10.5607/en25042

Figure Lengend Snippet: Diagram of acupoint selection for electroacupuncture treatment.

Article Snippet: Following separation by 10% SDS–PAGE (G2043, Servicebio, Wuhan, China) and transfer to PVDF (G6044, Servicebio, Wuhan, China) membranes, the blots were blocked with 5% BSA ( GC305010 , Servicebio, Wuhan, China) and probed overnight at 4°C with primary antibodies, including cGAS (1:2,000, 26416-1-AP, Proteintech, Wuhan, China), STING (1:2,000, 19851-1-AP, Proteintech, Wuhan, China), IRF3 (1:2,000, 11312-1-AP, Proteintech, Wuhan, China), NF-κB p65 (1:2,000, 10745-1-AP, Proteintech, Wuhan, China), and IL-1β (1:2,000, 26048-1-AP, Proteintech, Wuhan, China) and α-Tubulin (1:10,000, GB11200, Servicebio, Wuhan, China).

Techniques: Selection

Journal: Experimental Neurobiology

Article Title: Electroacupuncture Attenuates Neuroinflammation and Postoperative Cognitive Dysfunction in Aged Rats by Suppressing the cGAS–STING Pathway

doi: 10.5607/en25042

Figure Lengend Snippet: Electroacupuncture downregulates the cGAS–STING pathway and attenuates neuroinflammatory signaling in the hippocampus. (A) Representative Western blot images showing hippocampal protein levels of cGAS, STING, NF-κB p65, IRF3, and IL-1β. (B~F) Quantitative analysis of protein expression normalized to α-Tubulin. (G, I) Immunofluorescence micrographs depicting hippocampal cGAS and STING expression across groups. (H, J) Quantification of mean fluorescence intensity for cGAS and STING. (n=5 biological replicates; *p<0.05, **p<0.01, ***p<0.001; ns, not statistically significant).

Article Snippet: Following separation by 10% SDS–PAGE (G2043, Servicebio, Wuhan, China) and transfer to PVDF (G6044, Servicebio, Wuhan, China) membranes, the blots were blocked with 5% BSA ( GC305010 , Servicebio, Wuhan, China) and probed overnight at 4°C with primary antibodies, including cGAS (1:2,000, 26416-1-AP, Proteintech, Wuhan, China), STING (1:2,000, 19851-1-AP, Proteintech, Wuhan, China), IRF3 (1:2,000, 11312-1-AP, Proteintech, Wuhan, China), NF-κB p65 (1:2,000, 10745-1-AP, Proteintech, Wuhan, China), and IL-1β (1:2,000, 26048-1-AP, Proteintech, Wuhan, China) and α-Tubulin (1:10,000, GB11200, Servicebio, Wuhan, China).

Techniques: Western Blot, Expressing, Immunofluorescence, Fluorescence

Journal: Experimental Neurobiology

Article Title: Electroacupuncture Attenuates Neuroinflammation and Postoperative Cognitive Dysfunction in Aged Rats by Suppressing the cGAS–STING Pathway

doi: 10.5607/en25042

Figure Lengend Snippet: RU.521 inhibits the activation of the cGAS-STING signaling pathway and its downstream molecules. (A) Representative Western blot images showing hippocampal of cGAS, STING, IRF3, NF-κB p65, and IL-1β protein levels. (B~F) Quantification of protein expression normalized to α-Tubulin. (G, I) Immunofluorescence micrographs depicting hippocampal cGAS and STING expression across experimental groups. (H, J) Quantification of mean fluorescence intensity for cGAS (H) and STING (J) (n=5 biological replicates; *p<0.05, **p<0.01, ***p<0.001; ns, not significant; EA, electroacupuncture).

Article Snippet: Following separation by 10% SDS–PAGE (G2043, Servicebio, Wuhan, China) and transfer to PVDF (G6044, Servicebio, Wuhan, China) membranes, the blots were blocked with 5% BSA ( GC305010 , Servicebio, Wuhan, China) and probed overnight at 4°C with primary antibodies, including cGAS (1:2,000, 26416-1-AP, Proteintech, Wuhan, China), STING (1:2,000, 19851-1-AP, Proteintech, Wuhan, China), IRF3 (1:2,000, 11312-1-AP, Proteintech, Wuhan, China), NF-κB p65 (1:2,000, 10745-1-AP, Proteintech, Wuhan, China), and IL-1β (1:2,000, 26048-1-AP, Proteintech, Wuhan, China) and α-Tubulin (1:10,000, GB11200, Servicebio, Wuhan, China).

Techniques: Activation Assay, Western Blot, Expressing, Immunofluorescence, Fluorescence